-111 hTF m1
(Plasmid
#15448)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL2 Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTissue factor promoter
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Alt nameTF
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SpeciesH. sapiens (human)
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Insert Size (bp)125
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Entrez GeneF3 (a.k.a. CD142, TF, TFA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
-111 to +14 hTF promoter. hTF promoter deletion/mutation series 2, Mackman lab #2428.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
-111 hTF m1 was a gift from Nigel Mackman (Addgene plasmid # 15448 ; http://n2t.net/addgene:15448 ; RRID:Addgene_15448) -
For your References section:
Transcriptional regulation of the tissue factor gene in human epithelial cells is mediated by Sp1 and EGR-1. Cui MZ, Parry GC, Oeth P, Larson H, Smith M, Huang RP, Adamson ED, Mackman N. J Biol Chem. 1996 Feb 2. 271(5):2731-9. 10.1074/jbc.271.5.2731 PubMed 8576248