MSCV-Stop-EGFP (MSE)
(Plasmid
#154469)
-
Purpose(Empty Backbone) MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 154469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneMSCV
- Backbone size (bp) 6792
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCV-Stop-EGFP (MSE) was a gift from Cornelius Miething (Addgene plasmid # 154469 ; http://n2t.net/addgene:154469 ; RRID:Addgene_154469) -
For your References section:
A novel conditional NPM-ALK-driven model of CD30+ T-cell lymphoma mediated by a translational stop cassette. Shoumariyeh K, Schneider N, Poggio T, Veratti P, Ehrenfeld S, Redhaber DM, Khan R, Pfeifer D, Klingeberg C, Kreutmair S, Rudelius M, Quintanilla-Martinez L, Fend F, Illert AL, Duyster J, Miething C. Oncogene. 2020 Feb;39(9):1904-1913. doi: 10.1038/s41388-019-1058-1. Epub 2019 Nov 21. 10.1038/s41388-019-1058-1 PubMed 31754210