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pET15b T7pCONS TIR-2 sfGFP
(Plasmid #154468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    T7 promoter changed as well as the translation initiation region
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    super folder GFP
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • His-thrombin (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b T7pCONS TIR-2 sfGFP was a gift from Daniel Daley (Addgene plasmid # 154468 ; http://n2t.net/addgene:154468 ; RRID:Addgene_154468)

  • For your References section:

    Improved designs for pET expression plasmids increase protein production yield in Escherichia coli. Shilling PJ, Mirzadeh K, Cumming AJ, Widesheim M, Kock Z, Daley DO. Commun Biol. 2020 May 7;3(1):214. doi: 10.1038/s42003-020-0939-8. 10.1038/s42003-020-0939-8 PubMed 32382055
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