pET28a T7pCONS TIR-1 sfGFP
(Plasmid
#154463)
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PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesuper folder GFP
- Promoter T7
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Tag
/ Fusion Protein
- His-thrombin (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7p
- 3′ sequencing primer T7t (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a T7pCONS TIR-1 sfGFP was a gift from Daniel Daley (Addgene plasmid # 154463 ; http://n2t.net/addgene:154463 ; RRID:Addgene_154463) -
For your References section:
Improved designs for pET expression plasmids increase protein production yield in Escherichia coli. Shilling PJ, Mirzadeh K, Cumming AJ, Widesheim M, Kock Z, Daley DO. Commun Biol. 2020 May 7;3(1):214. doi: 10.1038/s42003-020-0939-8. 10.1038/s42003-020-0939-8 PubMed 32382055
