pCDNA5/mCherry_pBirA-HA_FKBP8
(Plasmid #154457)

• Purpose: expresses mCherry_pBirA-HA_FKBP8/biochemical assay
• Depositing Lab: Hyun-Woo Rhee
• Publication: Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 10.1073/pnas.1916584117.

Backbone

• Vector backbone: pCDNA5
• Backbone size w/o insert (bp): 5089
• Total vector size (bp): 8050
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry_pBirA-HA_FKBP8
• Species: H. sapiens (human)
• Insert Size (bp): 2961
• GenBank ID: NM_001308373.1
• Entrez Gene: FKBP8 (a.k.a. FKBP38, FKBPr38)
• Promoter: CMV-F

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII, HindIII, KpnI (not destroyed)
• 3′ cloning site: AscI, XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter, CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry