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Addgene

pcDNA5-BirA-FLAG-N-term-HDLBP
(Plasmid #154453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST_pcDNA5_Flag_N-term
  • Backbone manufacturer
    Gingras lab PMID: 24255178
  • Backbone size w/o insert (bp) 7838
  • Total vector size (bp) 9900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HDLBP
  • Alt name
    Vigilin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3807
  • Entrez Gene
    HDLBP (a.k.a. HBP, PRO2900, VGL)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-BirA-FLAG-N-term-HDLBP was a gift from Markus Landthaler (Addgene plasmid # 154453 ; http://n2t.net/addgene:154453 ; RRID:Addgene_154453)

  • For your References section:

    HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, Kempa S, Huttelmaier S, Selbach M, Landthaler M. Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7. 10.1038/s41467-022-30322-7 PubMed 35585045
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