pDONR221_HDLBP
(Plasmid
#154448)
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PurposeFor recombination of HDLBP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2550
- Total vector size (bp) 6357
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHDLBP
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Alt nameVigilin
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SpeciesH. sapiens (human)
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Insert Size (bp)3807
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Entrez GeneHDLBP (a.k.a. HBP, PRO2900, VGL)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_HDLBP was a gift from Markus Landthaler (Addgene plasmid # 154448 ; http://n2t.net/addgene:154448 ; RRID:Addgene_154448) -
For your References section:
HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, Kempa S, Huttelmaier S, Selbach M, Landthaler M. Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7. 10.1038/s41467-022-30322-7 PubMed 35585045