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pCDNA3/flag-pBirA(1-G78)-FRB
(Plasmid #154447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 5952
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flag-pBirA(1-G78)-FRB
  • Alt name
    split BioID(N-G78)-FRB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    570
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site Xhoi, XbaI (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3/flag-pBirA(1-G78)-FRB was a gift from Hyun-Woo Rhee (Addgene plasmid # 154447 ; http://n2t.net/addgene:154447 ; RRID:Addgene_154447)

  • For your References section:

    Contact-ID, a tool for profiling organelle contact sites, reveals regulatory proteins of mitochondrial-associated membrane formation. Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH, Park SK, Mun JY, Kim JS, Rhee HW. Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 10.1073/pnas.1916584117. 10.1073/pnas.1916584117 PubMed 32414919
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