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pET21-tVYN-Nt∆
(Plasmid #154431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7206
  • Modifications to backbone
    Inserted at NdeI/HindIII
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VenusΔC12-NtYcgRΔ-NLucΔ4
  • Alt name
    tVYN-NtΔ
  • Insert Size (bp)
    1824
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-tVYN-Nt∆ was a gift from Ming Hammond (Addgene plasmid # 154431 ; http://n2t.net/addgene:154431 ; RRID:Addgene_154431)

  • For your References section:

    Development of Ratiometric Bioluminescent Sensors for in Vivo Detection of Bacterial Signaling. Dippel AB, Anderson WA, Park JH, Yildiz FH, Hammond MC. ACS Chem Biol. 2020 Mar 25. doi: 10.1021/acschembio.9b00800. 10.1021/acschembio.9b00800 PubMed 32186367
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