hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
(Plasmid #154430)

• Purpose: hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
• Depositing Lab: David Liu
• Publication: Koblan et al Nature. 2021 Jan;589(7843):608-614. doi: 10.1038/s41586-020-03086-7. Epub 2021 Jan 6.

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Backbone

• Vector backbone: lentiCRISPRv2
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
• Species: H. sapiens (human)
• Mutation: VRQR point mutations in SpCas9

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector FOR ADDGENE.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry