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hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
(Plasmid #154429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPRv2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
  • Species
    H. sapiens (human)
  • Mutation
    VRQR point mutations in SpCas9

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid was a gift from David Liu (Addgene plasmid # 154429 ; http://n2t.net/addgene:154429 ; RRID:Addgene_154429)

  • For your References section:

    In vivo base editing rescues Hutchinson-Gilford progeria syndrome in mice. Koblan LW, Erdos MR, Wilson C, Cabral WA, Levy JM, Xiong ZM, Tavarez UL, Davison LM, Gete YG, Mao X, Newby GA, Doherty SP, Narisu N, Sheng Q, Krilow C, Lin CY, Gordon LB, Cao K, Collins FS, Brown JD, Liu DR. Nature. 2021 Jan;589(7843):608-614. doi: 10.1038/s41586-020-03086-7. Epub 2021 Jan 6. 10.1038/s41586-020-03086-7 PubMed 33408413
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