KRAS-V14I
(Plasmid #154427)

• Purpose: V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.
• Depositing Lab: Kenneth Westover
• Publication: Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-13972. doi: 10.1074/jbc.RA119.009131. Epub 2019 Jul 24.

Backbone

• Vector backbone: pcDNA2.0
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KRAS
• Alt name: Kirsten ras oncogene
• Species: H. sapiens (human)
• Insert Size (bp): 507
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Tags / Fusion Proteins:
  • 6xHis-tag
  • TEV protease cleavage sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry