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pDONR223 SARS-CoV2 TEV-NSP8
(Plasmid #154407)

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Item Catalog # Description Quantity Price (USD)
Plasmid 154407 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway-compatible Entry vector, with insert of NSP8 CDS with a TEV sequence at the N-term.

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NSP8
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
    621
  • Mutation
    Many synonymous changes due to codon optimization
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR amplified

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 SARS-CoV2 TEV-NSP8 was a gift from Fritz Roth (Addgene plasmid # 154407 ; http://n2t.net/addgene:154407 ; RRID:Addgene_154407)

  • For your References section:

    A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951
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