Skip to main content
Addgene

pDONR207 SARS-CoV2 TEV-NSP3
(Plasmid #154402)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154402 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pDONR207
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway-compatible Entry vector, with insert of NSP3 CDS with a TEV sequence at the N-term.

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NSP3
  • Alt name
    PLPRO
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
    5862
  • Mutation
    Many synonymous changes due to codon optimization
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR amplified

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR207 SARS-CoV2 TEV-NSP3 was a gift from Fritz Roth (Addgene plasmid # 154402 ; http://n2t.net/addgene:154402 ; RRID:Addgene_154402)

  • For your References section:

    A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951
Related items:
Commonly requested with: