pAH18
(Plasmid #154397)

• Purpose: Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
• Depositing Lab: Silvia Cardona
• Publication: Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232.

Backbone

• Vector backbone: pGPI-SceI
• Backbone manufacturer: Miguel Valvano
• Backbone size w/o insert (bp): 4748
• Total vector size (bp): 5620
• Modifications to backbone: None
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Trimethoprim
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: Trimethoprim 50 ug/mL
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
• Species: Synthetic; B. cenocepacia K56-2
• Insert Size (bp): 950
• GenBank ID: LAUA00000000.1
• Promoter: NA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pAH18.gb
• A portion of this plasmid was derived from a plasmid made by: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene sequence differs slightly from the author's sequence (.gb file) in non-coding regions of the plasmid. These differences do not alter the plasmid's function.