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pET32b-MED19
(Plasmid #15438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET32a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MED19
  • Alt name
    LCMR1
  • Alt name
    Rox3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    735
  • GenBank ID
    AK010552 AW044776
  • Entrez Gene
    Med19 (a.k.a. LCMR1, 2410018M14Rik, 3110040A13Rik, DKFZp586K0524)
  • Tags / Fusion Proteins
    • Trx (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Stag
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32b-MED19 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15438 ; http://n2t.net/addgene:15438 ; RRID:Addgene_15438)

  • For your References section:

    Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2003 Apr 25. 278(17):15123-7. 10.1074/jbc.C300054200 PubMed 12584197
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