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pSrTA1662-toolkit
(Plasmid #154372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2356
  • Total vector size (bp) 12817
  • Vector type
    Bacterial Expression, Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli K12 OmniMAX™ 2 T1R bacterial strain should be used for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SrTA1662
  • Species
    Aegilops tauschii
  • Insert Size (bp)
    10461
  • GenBank ID
    MG763911.1
  • Promoter Native promoter

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic SrTA1662 gene was assembled by Invitrogen (ThermoFisher) from synthetic oligonucleotides and/ or PCR products.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSrTA1662-toolkit was a gift from Brande Wulff (Addgene plasmid # 154372 ; http://n2t.net/addgene:154372 ; RRID:Addgene_154372)

  • For your References section:

    Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement. Gaurav K, Arora S, Silva P, Sanchez-Martin J, Horsnell R, Gao L, Brar GS, Widrig V, John Raupp W, Singh N, Wu S, Kale SM, Chinoy C, Nicholson P, Quiroz-Chavez J, Simmonds J, Hayta S, Smedley MA, Harwood W, Pearce S, Gilbert D, Kangara N, Gardener C, Forner-Martinez M, Liu J, Yu G, Boden SA, Pascucci A, Ghosh S, Hafeez AN, O'Hara T, Waites J, Cheema J, Steuernagel B, Patpour M, Justesen AF, Liu S, Rudd JC, Avni R, Sharon A, Steiner B, Kirana RP, Buerstmayr H, Mehrabi AA, Nasyrova FY, Chayut N, Matny O, Steffenson BJ, Sandhu N, Chhuneja P, Lagudah E, Elkot AF, Tyrrell S, Bian X, Davey RP, Simonsen M, Schauser L, Tiwari VK, Randy Kutcher H, Hucl P, Li A, Liu DC, Mao L, Xu S, Brown-Guedira G, Faris J, Dvorak J, Luo MC, Krasileva K, Lux T, Artmeier S, Mayer KFX, Uauy C, Mascher M, Bentley AR, Keller B, Poland J, Wulff BBH. Nat Biotechnol. 2022 Mar;40(3):422-431. doi: 10.1038/s41587-021-01058-4. Epub 2021 Nov 1. 10.1038/s41587-021-01058-4 PubMed 34725503
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