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Addgene

pJD427
(Plasmid #154371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB3C5
  • Backbone manufacturer
    biobrick
  • Backbone size w/o insert (bp) 2738
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
  • Alt name
    constitutive production of split-adapter system (rapamycin induced dimerization)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJD427 was a gift from Joseph Davis (Addgene plasmid # 154371 ; http://n2t.net/addgene:154371 ; RRID:Addgene_154371)

  • For your References section:

    Small-molecule control of protein degradation using split adaptors. Davis JH, Baker TA, Sauer RT. ACS Chem Biol. 2011 Nov 18;6(11):1205-13. doi: 10.1021/cb2001389. Epub 2011 Sep 8. 10.1021/cb2001389 PubMed 21866931
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