pMAL-C2X dAmp/Kana+-2AFP
(Plasmid
#154358)
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PurposeBacterial expression of 2AFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAL-c2X
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name2AFP
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SpeciesUnspecified
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL-C2X dAmp/Kana+-2AFP was a gift from Wanzhong He (Addgene plasmid # 154358 ; http://n2t.net/addgene:154358 ; RRID:Addgene_154358) -
For your References section:
Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells. Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W. Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z. Epub 2020 Aug 10. 10.1038/s41592-020-0911-z PubMed 32778831