pET41a-MED22
(Plasmid #15432)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-7.

Backbone

• Vector backbone: pET41a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MED22
• Alt name: Surf5b
• Alt name: AJ224359
• Alt name: SRB6
• Species: H. sapiens (human)
• Insert Size (bp): 603
• Mutation: N/A
• GenBank ID: AJ224359
• Entrez Gene: MED22 (a.k.a. RP11-244N20.9, MED24, SURF5, surf-5)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry