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pENTR1A mKate2-hu_ncOGT 5N5A
(Plasmid #154285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Campeau et al. PLOS One 2009
  • Backbone size w/o insert (bp) 2300
  • Total vector size (bp) 6176
  • Vector type
    Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stellar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    O-GlcNAc Transferase
  • Alt name
    OGT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3903
  • Mutation
    N322A, N356A, N390A, N424A, N458A mutations (HCF-1 cleavage impaired)
  • GenBank ID
    NM_181673.3
  • Entrez Gene
    OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
  • Tags / Fusion Proteins
    • mKate2 (N terminal on insert)
    • 2x FLAG (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.10.22.351288 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1A mKate2-hu_ncOGT 5N5A was a gift from Suzanne Walker (Addgene plasmid # 154285 ; http://n2t.net/addgene:154285 ; RRID:Addgene_154285)

  • For your References section:

    Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 10.1073/pnas.2016778118. 10.1073/pnas.2016778118 PubMed 33419956
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