pBJG1 T7-8xHis-OGT 5N5A
(Plasmid #154280)

• Purpose: Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
• Depositing Lab: Suzanne Walker
• Publication: Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-3513. doi: 10.1021/jacs.7b13546. Epub 2018 Mar 5.

Backbone

• Vector backbone: pET24b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 8412
• Modifications to backbone: subcloned MCS from alternate pET vector to take tag as described in SI of Gross et al., JACS 2005
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: O-GlcNAc Transferase
• Alt name: OGT
• Species: H. sapiens (human)
• Insert Size (bp): 3135
• Mutation: N322A, N356A, N390A, N424A, N458A mutations; bacterial codon optimized
• Entrez Gene: OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
• Tags / Fusion Proteins:
  • T7 leader (N terminal on insert)
  • 8x His (N terminal on insert)
  • HRV3C protease site (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pBJG1 T7-8xHis-HRC3V-OGT 5N5A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry