pBJG1 T7-8xHis-OGT K842M
(Plasmid #154272)

• Purpose: Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
• Depositing Lab: Suzanne Walker
• Publication: Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi: 10.1038/nature09638. Epub 2011 Jan 16.

Backbone

• Vector backbone: pET24b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 8412
• Modifications to backbone: subcloned MCS from alternate pET vector to take tag as described in SI of Gross et al., JACS 2005
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NovaBlue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: O-GlcNAc Transferase
• Alt name: OGT
• Species: H. sapiens (human)
• Insert Size (bp): 3135
• Mutation: Lysine 842 changed to Methionine- bacterial codon optimized
• Entrez Gene: OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
• Tags / Fusion Proteins:
  • T7 leader (N terminal on insert)
  • 8x His (N terminal on insert)
  • HRV3C protease site (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pBJG1 T7-8xHis-HRC3V-OGT K842M.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry