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Addgene

SARS-CoV Nucleocapsid
(Plasmid #154269)

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Item Catalog # Description Quantity Price (USD)
Plasmid 154269 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone manufacturer
    Rupp and Turner
  • Backbone size w/o insert (bp) 4352
  • Total vector size (bp) 5580
  • Modifications to backbone
    deleted part of polylinker from NcoI to XbaI site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV Nucleocapsid
  • Alt name
    myc-tagged nucleocapsid from SARS-CoV (1).
  • Species
    SARS coronavirus Urbani isolate icSARS-C7-MA
  • Insert Size (bp)
    1305
  • GenBank ID
    MK062184.1
  • Promoter CMV
  • Tag / Fusion Protein
    • 5X myc epitope (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV Nucleocapsid was a gift from Peter Klein (Addgene plasmid # 154269 ; http://n2t.net/addgene:154269 ; RRID:Addgene_154269)
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