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pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc
(Plasmid #154265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-TRE3G-MCS_PGK-GpNLuc
  • Backbone manufacturer
    custom (Amelio Lab)
  • Backbone size w/o insert (bp) 7193
  • Total vector size (bp) 10307
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-CRTC1/MAML2 fusion oncogene
  • Alt name
    C1/M2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3114
  • Promoter TRE3G
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TRE3G-gatcgcctggagcaattccac
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc was a gift from Antonio Amelio (Addgene plasmid # 154265 ; http://n2t.net/addgene:154265 ; RRID:Addgene_154265)

  • For your References section:

    CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers vulnerability to PPARgamma inhibition. Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL. Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768. 10.1016/j.celrep.2021.108768 PubMed 33626346
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