pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro
(Plasmid #154263)

• Purpose: Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.
• Depositing Lab: Antonio Amelio
• Publication: Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768.

Backbone

• Vector backbone: pLV
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 9054
• Total vector size (bp): 9852
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xFLAG-PGC-1a4 splice variant
• Alt name: PPARGC1A
• Species: H. sapiens (human)
• Insert Size (bp): 798
• GenBank ID: Gene ID: 10891
• Entrez Gene: PPARGC1A (a.k.a. LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1)
• Promoter: TRE3G
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TRE3G-gatcgcctggagcaattccac

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry