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pPGC1a_Proximal Promoter-FLuc2P
(Plasmid #154259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.15
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5969
  • Total vector size (bp) 6469
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1a Proximal Promoter
  • Alt name
    PPARGC1A Proximal Promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • GenBank ID
    Gene ID: 10891
  • Entrez Gene
    PPARGC1A (a.k.a. LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1)
  • Promoter Human PGC-1alpha Proximal Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPGC1a_Proximal Promoter-FLuc2P was a gift from Antonio Amelio (Addgene plasmid # 154259 ; http://n2t.net/addgene:154259 ; RRID:Addgene_154259)

  • For your References section:

    CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers vulnerability to PPARgamma inhibition. Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL. Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768. 10.1016/j.celrep.2021.108768 PubMed 33626346
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