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Addgene

FLAG-MED27
(Plasmid #15424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 N-FLAG/Hyg
  • Backbone manufacturer
    Invitrogen, modified at Conaway lab
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MED27
  • Alt name
    CRSP34
  • Alt name
    CRSP8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    822
  • Mutation
    N/A
  • GenBank ID
    NM_004269
  • Entrez Gene
    MED27 (a.k.a. CRAP34, CRSP34, CRSP8, MED3, TRAP37)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-MED27 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15424 ; http://n2t.net/addgene:15424 ; RRID:Addgene_15424)

  • For your References section:

    Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2003 Apr 25. 278(17):15123-7. 10.1074/jbc.C300054200 PubMed 12584197
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