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pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS
(Plasmid #154231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM4673
  • Backbone manufacturer
    MoClo Tool Kit of Sylvestre Marillonnet
  • Backbone size w/o insert (bp) 8105
  • Vector type
    Bacterial Expression, Synthetic Biology ; Fungal Expression
  • Selectable markers
    amdS fungal selection

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p40S:QfDBD-VP16AD-GFP-NLS
  • Alt name
    P(various):DsRed-SKL
  • Alt name
    pGpdA:amdS
  • Species
    Synthetic; Fungal expression, P. rubens (formerly P. chrysogenum)
  • Promoter p40S (AN0465 A. nidulans)
  • Tag / Fusion Protein
    • GFP-NLS; DsRed-SKL (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS was a gift from Arnold Driessen (Addgene plasmid # 154231 ; http://n2t.net/addgene:154231 ; RRID:Addgene_154231)

  • For your References section:

    Synthetic control devices for gene regulation in Penicillium chrysogenum. Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y. Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3. 10.1186/s12934-019-1253-3 PubMed 31739777
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