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Addgene

pET-11a HIV-1 P6
(Plasmid #154201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5637
  • Total vector size (bp) 6060
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gag P6 protein from HIV-1 clone HXB2
  • Insert Size (bp)
    423
  • Promoter T7
  • Tag / Fusion Protein
    • GB1-6xHis Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-11a HIV-1 P6 was a gift from Lalit Deshmukh (Addgene plasmid # 154201 ; http://n2t.net/addgene:154201 ; RRID:Addgene_154201)

  • For your References section:

    Quantitative NMR Study of Insulin-Degrading Enzyme Using Amyloid-beta and HIV-1 p6 Elucidates Its Chaperone Activity. Ramaraju B, Nelson SL, Zheng W, Ghirlando R, Deshmukh L. Biochemistry. 2021 Aug 3. doi: 10.1021/acs.biochem.1c00342. 10.1021/acs.biochem.1c00342 PubMed 34342986
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