CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C
(Plasmid #154197)

• Purpose: Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
• Depositing Lab: Anna Obenauf
• Publication: Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 10.1038/s41587-020-0614-0.

Backbone

• Vector backbone: pRRL
• Vector type: Mammalian Expression, Lentiviral ; CaTCH barcode activation
• Selectable markers: Neomycin (select with G418) ; Thy1.1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sgRNA-BC2-A, sgRNA-BC2-C
• gRNA/shRNA sequence: GTAGGAAACTTCAAATACCA, GAGGTCTGGAATATCAGCC
• Species: Synthetic
• Promoter: hU6 and mU6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C.gb
• A portion of this plasmid was derived from a plasmid made by: Johannes Zuber at Institute of Molecular Pathology, Vienna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry