dCas9-VPR_P2A_mCherry
(Plasmid #154193)

• Purpose: Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker
• Depositing Lab: Anna Obenauf
• Publication: Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 10.1038/s41587-020-0614-0.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 4507
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sp-dCas9-VPR_P2A_mCherry
• Species: Synthetic
• Insert Size (bp): 6579
• Mutation: D10A, H840A
• Promoter: EF1as

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • dCas9-VPR_P2A_mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Johannes Zuber at Institute of Molecular Pathology, Vienna
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry