pMAL-p2x-LanCL2
(Plasmid
#154192)
-
PurposeTo express MBP-LanCL2 in bacterial cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 154192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMAL-p2x
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameLanCL2
-
Alt nameLanC-like protein 2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1350
-
GenBank ID
-
Tag
/ Fusion Protein
- Maltose binding protein (MBP) (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer 5'-GGTCGTCAGACTGTCGATGAAGCC-3' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMAL-p2x-LanCL2 was a gift from Wilfred van der Donk (Addgene plasmid # 154192 ; http://n2t.net/addgene:154192 ; RRID:Addgene_154192) -
For your References section:
LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome. Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair SK, Chen J, Davis BG, van der Donk WA. Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 10.1016/j.cell.2021.04.001. 10.1016/j.cell.2021.04.001 PubMed 33932340