FLAG-MED18
(Plasmid #15419)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-7.

Backbone

• Vector backbone: pcDNA3.1 N-FLAG/Hyg
• Backbone manufacturer: Invitrogen, modified at Conaway lab
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MED18
• Alt name: p28b
• Alt name: Srb5
• Species: H. sapiens (human)
• Insert Size (bp): 627
• Mutation: N/A
• GenBank ID: AA460637
• Entrez Gene: MED18 (a.k.a. SRB5, p28b)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry