pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
(Plasmid
#154185)
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Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF-GFP
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Backbone manufacturer#11154
- Backbone size w/o insert (bp) 1668
- Total vector size (bp) 5985
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Modifications to backbonedeleted GFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameretroCHMP3
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SpeciesS. sciureus (squirrel monkey)
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Insert Size (bp)495
- Promoter EF1-alpha
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Tags
/ Fusion Proteins
- HA (C terminal on insert)
- T2A-H2B-BFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ggaatttgccctttttgagtttgg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKate Bredbenner, Sanford M. Simon laboratory, Rockefeller University
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP was a gift from Wesley Sundquist (Addgene plasmid # 154185 ; http://n2t.net/addgene:154185 ; RRID:Addgene_154185) -
For your References section:
RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis. Rheinemann L, Downhour DM, Bredbenner K, Mercenne G, Davenport KA, Schmitt PT, Necessary CR, McCullough J, Schmitt AP, Simon SM, Sundquist WI, Elde NC. Cell. 2021 Sep 27. pii: S0092-8674(21)01055-2. doi: 10.1016/j.cell.2021.09.008. 10.1016/j.cell.2021.09.008 PubMed 34597582