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Addgene

pKM33
(Plasmid #154167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154167 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIEx
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rel2 (long isoform)
  • Species
    Other
  • Mutation
    C-terminal IκB domain deleted
  • Promoter IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM33 was a gift from Kevin Maringer (Addgene plasmid # 154167 ; http://n2t.net/addgene:154167 ; RRID:Addgene_154167)

  • For your References section:

    Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells. Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K. PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. 10.1371/journal.pntd.0008524 PubMed 33591970
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