pAA3
(Plasmid #154163)

• Purpose: Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
• Depositing Lab: Kevin Maringer
• Publication: Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524.

Backbone

• Vector backbone: pLUC-MCS
• Modifications to backbone: 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-κB sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-κB site.
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Species: Other
• Mutation: 20 wt NF-κB sites were replaced with Rel1A-specific NF-κB sites

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pAA3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry