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Addgene

pKM62
(Plasmid #154160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLUC-MCS
  • Modifications to backbone
    Enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-κB sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-κB site.
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Species
    Other
  • Mutation
    4 wt NF-κB sites were replaced with Rel2-specific (VII) NF-κB sites

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM62 was a gift from Kevin Maringer (Addgene plasmid # 154160 ; http://n2t.net/addgene:154160 ; RRID:Addgene_154160)

  • For your References section:

    Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells. Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K. PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. 10.1371/journal.pntd.0008524 PubMed 33591970
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