pKM54
(Plasmid
#154157)
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PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLUC-MCS
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly luciferase
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SpeciesOther
- Promoter 8 copies of the Aedes aegypti Rel2 NF-κB site
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKM54 was a gift from Kevin Maringer (Addgene plasmid # 154157 ; http://n2t.net/addgene:154157 ; RRID:Addgene_154157) -
For your References section:
Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells. Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K. PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. 10.1371/journal.pntd.0008524 PubMed 33591970