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PurposeTo detect the calcium ion releases close to the lysosomal surface
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBoBi
- Backbone size w/o insert (bp) 8300
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP2 and GCamp6s
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SpeciesH. sapiens (human)
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Insert Size (bp)2580
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MutationWT
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Entrez GeneLAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBoBi-hLAMP2-C-GC6s was a gift from Sheng-cai Lin (Addgene plasmid # 154151 ; http://n2t.net/addgene:154151 ; RRID:Addgene_154151) -
For your References section:
Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou Z, Xie XS, Hardie DG, Lin SC. Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13. 10.1016/j.cmet.2019.05.018 PubMed 31204282