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pBoBi-hTRPV4-C-GC6s
(Plasmid #154150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBoBi
  • Backbone size w/o insert (bp) 8300
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPV4 and GCamp6s
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3963
  • Mutation
    WT
  • Entrez Gene
    TRPV4 (a.k.a. BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains 5xHis at the N-terminus of GCaMP6s rather than 6xHis. This change does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBoBi-hTRPV4-C-GC6s was a gift from Sheng-cai Lin (Addgene plasmid # 154150 ; http://n2t.net/addgene:154150 ; RRID:Addgene_154150)

  • For your References section:

    Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou Z, Xie XS, Hardie DG, Lin SC. Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13. 10.1016/j.cmet.2019.05.018 PubMed 31204282
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