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Purpose(Empty Backbone) Gateway compatible mammalian expression plasmid for halo tag fusion proteins
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-Halo tag
- Backbone size (bp) 7412
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- Halo tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-Halo tag was a gift from Michael Huen (Addgene plasmid # 154144 ; http://n2t.net/addgene:154144 ; RRID:Addgene_154144)
