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Addgene

ptRNA100
(Plasmid #154133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Synthetic
  • Backbone size w/o insert (bp) 3366
  • Total vector size (bp) 4035
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli K12 JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNAs operon under control of Ptac promoter
  • Species
    Synthetic
  • Insert Size (bp)
    669

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptRNA100 was a gift from Alexander Mankin (Addgene plasmid # 154133 ; http://n2t.net/addgene:154133 ; RRID:Addgene_154133)

  • For your References section:

    Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS. Nat Commun. 2020 Jun 9;11(1):2900. doi: 10.1038/s41467-020-16694-8. 10.1038/s41467-020-16694-8 PubMed 32518240
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