pDH42(CUG/A)
(Plasmid
#154132)
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PurposeExpresses engineered (23S-5S phusion) rRNAs operon in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSynthetic
- Backbone size w/o insert (bp) 1836
- Total vector size (bp) 7461
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E. coli K12 POP2136
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameengineered bacterial rRNAs operon under control of pL promoter
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SpeciesSynthetic
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Insert Size (bp)5625
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDH42(CUG/A) was a gift from Alexander Mankin (Addgene plasmid # 154132 ; http://n2t.net/addgene:154132 ; RRID:Addgene_154132) -
For your References section:
Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS. Nat Commun. 2020 Jun 9;11(1):2900. doi: 10.1038/s41467-020-16694-8. 10.1038/s41467-020-16694-8 PubMed 32518240