poRFP-oGFP
(Plasmid #154130)

• Purpose: Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells
• Depositing Lab: Alexander Mankin
• Publication: Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi: 10.1038/s41467-020-15756-1.

Backbone

• Vector backbone: Synthetic
• Backbone size w/o insert (bp): 3448
• Total vector size (bp): 5659
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli K12 JM109
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: orthogonal gfp
• Species: Synthetic
• Insert Size (bp): 1268

Gene/Insert 2

• Gene/Insert name: orthogonal rfp
• Species: Synthetic
• Insert Size (bp): 943

Resource Information

• Supplemental Documents:
  • poRFP-oGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis identified multiple sequence discrepancies, relative to the sequence provided by the depositing laboratory. The depositing laboratory has indicated that these are not a concern for plasmid function.