poLuc1
(Plasmid
#154128)
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PurposeExpresses orthogonal firefly luciferase gene in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSynthetic
- Backbone size w/o insert (bp) 4354
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli K12 JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameorthogonal firefly luciferase
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SpeciesSynthetic
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Insert Size (bp)2212
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC NGS analysis identified multiple sequence discrepancies, relative to the sequence provided by the depositing laboratory. The depositing laboratory has indicated that these are not a concern for plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
poLuc1 was a gift from Alexander Mankin (Addgene plasmid # 154128 ; http://n2t.net/addgene:154128 ; RRID:Addgene_154128) -
For your References section:
A fully orthogonal system for protein synthesis in bacterial cells. Aleksashin NA, Szal T, d'Aquino AE, Jewett MC, Vazquez-Laslop N, Mankin AS. Nat Commun. 2020 Apr 20;11(1):1858. doi: 10.1038/s41467-020-15756-1. 10.1038/s41467-020-15756-1 PubMed 32313034
