poGFP1
(Plasmid
#154127)
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PurposeExpresses orthogonal gfp gene in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSynthetic
- Backbone size w/o insert (bp) 4354
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli K12 JM109
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
poGFP1 was a gift from Alexander Mankin (Addgene plasmid # 154127 ; http://n2t.net/addgene:154127 ; RRID:Addgene_154127) -
For your References section:
A fully orthogonal system for protein synthesis in bacterial cells. Aleksashin NA, Szal T, d'Aquino AE, Jewett MC, Vazquez-Laslop N, Mankin AS. Nat Commun. 2020 Apr 20;11(1):1858. doi: 10.1038/s41467-020-15756-1. 10.1038/s41467-020-15756-1 PubMed 32313034