pRBS wt
(Plasmid
#154126)
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PurposeExpresses wt rRNAs operon in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSynthetic
- Backbone size w/o insert (bp) 3414
- Total vector size (bp) 9029
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E. coli K12 POP2136
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namewt bacterial rRNAs operon under control of pL promotor
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SpeciesSynthetic
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Insert Size (bp)5615
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRBS wt was a gift from Alexander Mankin (Addgene plasmid # 154126 ; http://n2t.net/addgene:154126 ; RRID:Addgene_154126) -
For your References section:
A fully orthogonal system for protein synthesis in bacterial cells. Aleksashin NA, Szal T, d'Aquino AE, Jewett MC, Vazquez-Laslop N, Mankin AS. Nat Commun. 2020 Apr 20;11(1):1858. doi: 10.1038/s41467-020-15756-1. 10.1038/s41467-020-15756-1 PubMed 32313034
Map uploaded by the depositor.
