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Addgene

poRBS
(Plasmid #154125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Synthetic
  • Backbone size w/o insert (bp) 3414
  • Total vector size (bp) 9029
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    E. coli K12 POP2136
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    engineered bacterial rRNAs operon under control of pL promotor
  • Species
    Synthetic
  • Insert Size (bp)
    5615

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    poRBS was a gift from Alexander Mankin (Addgene plasmid # 154125 ; http://n2t.net/addgene:154125 ; RRID:Addgene_154125)

  • For your References section:

    A fully orthogonal system for protein synthesis in bacterial cells. Aleksashin NA, Szal T, d'Aquino AE, Jewett MC, Vazquez-Laslop N, Mankin AS. Nat Commun. 2020 Apr 20;11(1):1858. doi: 10.1038/s41467-020-15756-1. 10.1038/s41467-020-15756-1 PubMed 32313034
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