pNM3823
(Plasmid #154123)

• Purpose: mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector
• Depositing Lab: Michael Nonet
• Publication: Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.

Backbone

• Vector backbone: pLF3FShC
• Backbone manufacturer: Addgene #153083
• Backbone size w/o insert (bp): 7855
• Total vector size (bp): 10251
• Modifications to backbone: none
• Vector type: Worm Expression ; RMCE
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mec-4p::tetR-L-QFAD::act-4 '3
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 2500
• Promoter: not applicable

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: not applicable

Resource Information

• Supplemental Documents:
  • pNM3823.gb
• A portion of this plasmid was derived from a plasmid made by: The insert was added using a multi-fragment Golden Gate reaction with the mec-4p and act-4 3' UTR coming from amplified genomic sequences , the partially codon optimized tetR DNA binding domain, the flexible linker and codon optimized QF activation domain from synthetic DNA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry